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NCAM-1/CD56 Antibody (SPM128) [CoraFluor™ 1]

 
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Product Details

Summary
Reactivity Hu, Rt, ZeSpecies Glossary
Applications Simple Western, Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM128
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34397AF350
Alexa Fluor 405
NBP2-34397AF405
Alexa Fluor 488
NBP2-34397AF488
Alexa Fluor 532
NBP2-34397AF532
Alexa Fluor 594
NBP2-34397AF594
Alexa Fluor 647
NBP2-34397AF647
Alexa Fluor 700
NBP2-34397AF700
Alexa Fluor 750
NBP2-34397AF750
Allophycocyanin
NBP2-34397APC
Biotin
NBP2-34397B
DyLight 350
NBP2-34397UV
DyLight 405
NBP2-34397V
DyLight 488
NBP2-34397G
DyLight 550
NBP2-34397R
DyLight 594
NBP2-34397DL594
DyLight 650
NBP2-34397C
DyLight 680
NBP2-34397FR
DyLight 755
NBP2-34397IR
FITC
NBP2-34397F
HRP
NBP2-34397H
Janelia Fluor 525
NBP2-34397JF525
Janelia Fluor 549
NBP2-34397JF549
Janelia Fluor 585
NBP2-34397JF585
Janelia Fluor 635
NBP2-34397JF635
Janelia Fluor 646
NBP2-34397JF646
Janelia Fluor 669
NBP2-34397JF669
PE
NBP2-34397PE
PerCP
NBP2-34397PCP
Unconjugated
NBP2-34349-0.1mg
mFluor Violet 450 SE
NBP2-34397MFV450
mFluor Violet 500 SE
NBP2-34397MFV500
mFluor Violet 610 SE
NBP2-34397MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-34349-0.1mg
IHC-Prediluted
NBP2-44712
Azide and BSA Free
NBP2-34397-0.1mg
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NCAM-1/CD56 Antibody (SPM128) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Membrane preparation of a small cell lung carcinoma
Localization
Cell surface
Marker
Neuronal Cell Marker
Specificity
This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NCAM-1/CD56 Antibody (SPM128) [CoraFluor™ 1]

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NCAM-1/CD56
Antibody (SPM128) [Co ...
NBP2-34397CL1
NCAM-1/CD56 Antibody (SPM128) [CoraFluor™ 1]
Species: Hu, Rt, Ze
Applications: Simple Western, Flow, ICC/IF, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol NCAM1