Novus Biologicals products are now on bio-techne.com

NAPE-PLD Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: NAPE-PLD Antibody [NBP2-48571] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NAPE-PLD Antibody [NBP2-48571] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NAPE-PLD Antibody [NBP2-48571] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NAPE-PLD Antibody [NBP2-48571] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NAPE-PLD Antibody [NBP2-48571] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

NAPE-PLD Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ELPVLKPYFITNPEEAGVREAGLRVTWLGHATVMVEMDELIFLTDPIFSSRASPSQYMGPKRFRRSPCTISELPPIDAVLISHNHYDHLDYNSV
Predicted Species
Mouse (90%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAPEPLD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NAPE-PLD Recombinant Protein Antigen (NBP2-48571PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NAPE-PLD Antibody

  • C7orf18
  • DKFZp781D1098
  • EC 3.1.4
  • EC 3.1.4.-
  • EC 3.1.4.54
  • N-acyl phosphatidylethanolamine phospholipase DNAPE-PLDFMP30
  • N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
  • NAPE-hydrolyzing phospholipase D
  • NAPEPLD
  • NAPE-PLD

Background

NAPE-PLD (also known as N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D) is a membrane-bound phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine. NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes. Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00002166-M07
Species: Hu, Mu, Rt
Applications: ELISA, WB
NBP2-45564
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NLS32
Species: Bt, Bv, Ca, Eq, Hu, Pm, Mu, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-00735
Species: Hu, Pm
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00001269-M01
Species: Pm, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-71774
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
AF7197
Species: Hu, Mu
Applications: ICC, IHC, WB
AF4888
Species: Hu, Mu
Applications: CyTOF-ready, Flow, WB
AF4886
Species: Mu
Applications: IP, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP1-89783
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-689
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-22106
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
AF6868
Species: Hu
Applications: IHC, KO, WB
H00005706-M02
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, KD, WB
NB110-60930
Species: Hu, Mu
Applications: WB
H00151056-B01P
Species: Hu, Mu
Applications: WB
NBP3-17273
Species: Hu
Applications: ICC/IF, WB
DRP300
Species: Hu
Applications: ELISA
NBP2-48571
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC

Publications for NAPE-PLD Antibody (NBP2-48571) (0)

There are no publications for NAPE-PLD Antibody (NBP2-48571).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NAPE-PLD Antibody (NBP2-48571) (0)

There are no reviews for NAPE-PLD Antibody (NBP2-48571). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for NAPE-PLD Antibody (NBP2-48571) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NAPE-PLD Products

Research Areas for NAPE-PLD Antibody (NBP2-48571)

Find related products by research area.

Blogs on NAPE-PLD

There are no specific blogs for NAPE-PLD, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NAPE-PLD Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NAPEPLD