Orthogonal Strategies: Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues . Corresponding MYO5B RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium, placenta, skeletal muscle and small intestine using Anti-MYO5B antibody NBP1-87746 (A) shows ...read more
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
Simple Western: Myosin VB Antibody [NBP1-87746] - Analysis in RT4 at 0.2 mg/mL.
This antibody was developed against Recombinant Protein corresponding to amino acids: NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYO5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 27229121)
Immunohistochemistry 1:200 - 1:500
Immunohistochemistry-Paraffin 1:200 - 1:500
Knockout Validated Validation (PMID: 31664880)
Simple Western 1:100
Western Blot Reported in scientific literature (PMID: 27229121)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Mouse reactivity reported in scientific literature (PMID: 26201991). Use in Rat reported in scientific literature (PMID:31757887).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Myosin VB Antibody
KIAA1119
MYO5B variant protein
myosin VB
myosin-Vb
Background
Myosin and Kinesin motor domain. These ATPases belong to the P-loop NTPase family and provide the driving force in myosin and kinesin mediated processes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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![Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues . Corresponding MYO5B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myosin-VB-Antibody-Immunohistochemistry-Paraffin-NBP1-87746-img0024.jpg "Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues . Corresponding MYO5B RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium, placenta, skeletal muscle and small intestine using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein distribution across tissues to independent antibody NBP1-87747 (B).](http://images.novusbio.com/fullsize/Myosin-VB-Antibody-Immunohistochemistry-Paraffin-NBP1-87746-img0033.jpg "Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium, placenta, skeletal muscle and small intestine using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein distribution across tissues to independent antibody NBP1-87747 (B).")
![Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Myosin-VB-Antibody-Immunohistochemistry-Paraffin-NBP1-87746-img0020.jpg "Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Myosin-VB-Antibody-Immunohistochemistry-Paraffin-NBP1-87746-img0021.jpg "Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Myosin-VB-Antibody-Immunohistochemistry-Paraffin-NBP1-87746-img0022.jpg "Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Myosin-VB-Antibody-Immunohistochemistry-Paraffin-NBP1-87746-img0034.jpg "Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.")
![Simple Western: Myosin VB Antibody [NBP1-87746] - Analysis in RT4 at 0.2 mg/mL.](http://images.novusbio.com/fullsize/Myosin-VB-Antibody-Simple-Western-NBP1-87746-img0031.jpg "Simple Western: Myosin VB Antibody [NBP1-87746] - Analysis in RT4 at 0.2 mg/mL.")
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![Immunohistochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunohistochemistry_22971.jpg)
![Immunohistochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunohistochemistry_22972.jpg)
![Immunocytochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunocytochemistry_9890.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")