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Myosin 1B Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Myosin 1B Antibody [NBP1-87739] - Staining of human cell line A-431 shows positivity in plasma membrane and cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli and in cells in tubules.
Western Blot: Myosin 1B Antibody [NBP1-87739] - (B) Western blot of Myo1b from freshly isolated ATII cells and ATII cells after 2 days of culture (0 and 2, respectively) to demonstrate expression of Myo1b in ATII cells. ...read more
Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.
Western Blot: Myosin 1B Antibody [NBP1-87739] - Lysates of mouse whole brain in different developmental stages. Antibody at 1:1000. Molecular weight of the protein is around 130-140 KDa. Image submitted by a verified ...read more
Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human liver shows moderate membranous positivity in hepatocytes.
Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.
Western Blot: Myosin 1B Antibody [NBP1-87739] - Expression & localization of Myo1b & Myo1c in ATII cells. (A) Semi-quantitative RT-PCR showed that Myo1b & Myo1c are among the highest expressed Myo1 isoforms in ATII ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Myosin 1B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLNKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFMDHEAESVLAVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTGIDQSVLERAFSFRTVEAKQEKVSTTLNVA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYO1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot Validated from a verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Myosin 1B Protein (NBP1-87739PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-87739 in the following applications:

Publications
Read Publications using
NBP1-87739 in the following applications:

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 25812521). Rat reactivity reported in the scientific literature (PMID: 26940917). Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myosin 1B Antibody

  • MMIa
  • MMI-alpha
  • MYH-1c
  • MYO1B variant protein
  • Myosin I alpha
  • myosin IB
  • myosin-I alpha
  • myosin-Ib
  • myr1

Background

Myosin 1B is a motor protein which is involved in neuronal development, membrane trafficking/dynamics and functions including cell migration, vesicular transport and neurite outgrowth. Myosin 1B is known to have interactions with other proteins such as MAP3K3, RIPK3, BRF2, EIF4A2 and CIAO1. Myosin 1B has been studied in relation to neuronitis, amebiasis and dysentery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin 1B Antibody (NBP1-87739)(4)

Review for Myosin 1B Antibody (NBP1-87739) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-87739:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Myosin 1B NBP1-87739
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5
reviewed by:
Luiz Alexandre Magno
WB Mouse 10/17/2018
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Summary

ApplicationWestern Blot
Sample TestedMouse brain
SpeciesMouse
LotA39461

Comments

Commentst works very well at 1:1,000 in the brain but I would also suggest trying lower dilutions in tissues with lower expression of this type of myosin.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Myosin 1B Antibody (NBP1-87739) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Myosin 1B Antibody (NBP1-87739)

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Blogs on Myosin 1B

There are no specific blogs for Myosin 1B, but you can read our latest blog posts.
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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Luiz Alexandre Magno
10/17/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MYO1B