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![Immunocytochemistry/Immunofluorescence: Myosin 1B Antibody [NBP1-87739] - Staining of human cell line A-431 shows positivity in plasma membrane and cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87739-img0004.jpg "Immunocytochemistry/Immunofluorescence: Myosin 1B Antibody [NBP1-87739] - Staining of human cell line A-431 shows positivity in plasma membrane and cell junctions. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli and in cells in tubules.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-87739-img0013.jpg)
![Western Blot: Myosin 1B Antibody [NBP1-87739] - (B) Western blot of Myo1b from freshly isolated ATII cells and ATII cells after 2 days of culture (0 and 2, respectively) to demonstrate expression of Myo1b in ATII cells. Ponceau S staining was used to control for equal protein loading (M, marker). Image collected and cropped by CiteAb from the following publication (//jcs.biologists.org/lookup/doi/10.1242/jcs.181313) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Western-Blot-NBP1-87739-img0018.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-87739-img0016.jpg)
![Western Blot: Myosin 1B Antibody [NBP1-87739] - Lysates of mouse whole brain in different developmental stages. Antibody at 1:1000. Molecular weight of the protein is around 130-140 KDa. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Western-Blot-NBP1-87739-img0017.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-87739-img0014.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-87739-img0015.jpg)
![Western Blot: Myosin 1B Antibody [NBP1-87739] - Expression & localization of Myo1b & Myo1c in ATII cells. (A) Semi-quantitative RT-PCR showed that Myo1b & Myo1c are among the highest expressed Myo1 isoforms in ATII cells. Data (mean±s.e.m.) obtained from three cell isolations & three experiments per isolation are shown relative to the expression of the housekeeping gene HMBS. Day 0, freshly isolated cells; Day 2, after 2 days in culture. (B) Western blot of Myo1b & Myo1c from freshly isolated ATII cells & ATII cells after 2 days of culture (0 & 2, respectively) to demonstrate expression of Myo1b & Myo1c in ATII cells. Ponceau S staining was used to control for equal protein loading (M, marker). (C) Colocalization of anti Myo1b & Myo1c staining with fused secretory vesicles marked by ABCa3 & phalloidin immunostaining. The ABCa3 transporter is a marker for secretory vesicle membrane in ATII cells. After ATP stimulation, some vesicles fused with the plasma membrane & acquired an actin coat, which was labeled by phalloidin staining (arrow). Scale bars: 10 µm. Insets show the enlarged view of the fused lamellar bodies. Scale bars: 1 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/jcs/article/doi/10.1242/jcs.181313/260175/The-role-of-myosin-1c-and-myosin-1b-for-surfactant), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87739_rabbit-polyclonal-myosin-1b-antibody-310202416114745.jpg)
![Western Blot: Myosin 1B Antibody [NBP2-38888] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line PC-3](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Western-Blot-NBP2-38888-img0002.jpg "Western Blot: Myosin 1B Antibody [NBP2-38888] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line PC-3")
![Immunocytochemistry/Immunofluorescence: Myosin 1B Antibody [NBP2-38888] - Staining of human cell line U-2 OS shows localization to plasma membrane.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38888-img0003.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP2-38888] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-38888-img0008.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP2-38888] - Staining of human liver shows moderate membranous positivity in hepatocytes and bile duct cells.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-38888-img0004.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP2-38888] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-38888-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP2-38888] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-38888-img0006.jpg)
![Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP2-38888] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-38888-img0007.jpg)
![Western Blot: Myosin 1B Antibody (S04-6D4) [NBP3-19708] - Western blot detection of Myosin 1B in C6, 3T3, Hela cell lysates using NBP3-19708 (1:1000 diluted). Predicted band size: 132kDa. Observed band size: 132kDa.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-S04-6D4-Western-Blot-NBP3-19708-img0002.jpg "Western Blot: Myosin 1B Antibody (S04-6D4) [NBP3-19708] - Western blot detection of Myosin 1B in C6, 3T3, Hela cell lysates using NBP3-19708 (1:1000 diluted). Predicted band size: 132kDa. Observed band size: 132kDa.")
![Immunohistochemistry-Paraffin: Myosin 1B Antibody (S04-6D4) [NBP3-19708] - Immunohistochemistry of Myosin 1B in paraffin-embedded Human lung cancer tissue using NBP3-19708 at dilution 1/50](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-S04-6D4-Immunohistochemistry-Paraffin-NBP3-19708-img0001.jpg)
![Western Blot: Myosin 1B Antibody [NBP1-97778] - (1ug/ml) Analysis of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Western-Blot-NBP1-97778-img0002.jpg "Western Blot: Myosin 1B Antibody [NBP1-97778] - (1ug/ml) Analysis of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Myosin 1B Antibody (JE65-01) [NBP3-32629] - Western blot analysis of Myosin 1B on different lysates with Rabbit anti-Myosin 1B antibody (NBP3-32629) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32629) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32629_rabbit-myosin-1b-mab-je65-01-278202415242617.jpg "Western Blot: Myosin 1B Antibody (JE65-01) [NBP3-32629] - Western blot analysis of Myosin 1B on different lysates with Rabbit anti-Myosin 1B antibody (NBP3-32629) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32629) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Myosin 1B Antibody (JE65-01) [NBP3-32629] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Myosin 1B antibody (NBP3-32629) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32629) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32629_rabbit-myosin-1b-mab-je65-01-298202414161878.jpg)
![Western Blot: Myosin 1B Antibody [NBP2-19453] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Myosin-1B-Antibody-Western-Blot-NBP2-19453-img0001.jpg "Western Blot: Myosin 1B Antibody [NBP2-19453] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:1000.")