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Destrin Antibody (OTI2F7)

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Western Blot: Destrin Antibody (2F7) [NBP2-00527] Analysis of extracts (35ug) from 9 different cell lines by using anti-Destrin monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Destrin Antibody (2F7) [NBP2-00527] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Destrin.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Human prostate tissue using anti-Destrin mouse monoclonal antibody.
Flow Cytometry: Destrin Antibody (2F7) [NBP2-00527] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Destrin antibody, and then ...read more
Western Blot: Destrin Antibody (2F7) [NBP2-00527] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Destrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Human endometrium tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Human Kidney tissue using anti-Destrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Human pancreas tissue using anti-Destrin mouse monoclonal antibody.
Flow Cytometry: Destrin Antibody (2F7) [NBP2-00527] - Analysis of Hela cells, using anti-Destrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Destrin Antibody (2F7) [NBP2-00527] - Analysis of Jurkat cells, using anti-Destrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

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Destrin Antibody (OTI2F7) Summary

Immunogen
Full length human recombinant protein of human DSTN (NP_006861) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DSTN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Destrin Antibody (OTI2F7)

  • ACTDPdestrin
  • Actin-depolymerizing factor
  • ADFbA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP))
  • bA462D18.2
  • destrin (actin depolymerizing factor)
  • DSN

Background

The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DSTN
Entrez