Reactivity | HuSpecies Glossary |
Applications | IHC, MA |
Clone | MYOD1/2075R |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Biotin |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Recombinant human full-length MyoD protein (Uniprot: P15172 ) |
Localization | Nuclear |
Marker | Rhabdomyosarcoma Marker |
Specificity | Recognizes a phospho-protein of 45kDa, identified as MyoD1. This monoclonal antibody does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Only nuclear staining should be considered as evidence of skeletal muscle differentiation. |
Isotype | IgG |
Clonality | Monoclonal |
Host | Rabbit |
Gene | MYOD1 |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. |
Buffer | PBS |
Preservative | 0.05% Sodium Azide |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MyoD Antibody (NBP2-75772B)Find related products by research area.
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Gene Symbol | MYOD1 |