MyoD Antibody (5.8A) [DyLight 488]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	ICC	Mouse	04/18/2017	View Summary Application Immunocytochemistry Sample Tested Mouse skeletal muscle Species Mouse Lot AB100411A102016G Comments Comments Sample Information Cell Lines Tested: mouse skeletal muscle-derived primary cell population Species: mouse Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 Fixation Solution and Conditions: 4% formaldehyde treatment for 20min at RT Blocking Solution: no-treatment because of using fluorescence conjugated antibody in this experiment Time and Temperature: - Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times Primary Antibody Diluent: 0.5% tritonX-100 in PBS Dilutions Tested: 1:50      Time and Temperature: overnight at 4℃ Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times Detection System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 488nm Emission Filter: 562nm

Array NB100-56511G

• MyoD Antibodies
• MyoD ELISA Kits
• MyoD Lysate
• MyoD Protein