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MyoD Antibody (5.8A) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, KD, KO
Clone
5.8A
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56511AF350
Alexa Fluor 405
NB100-56511AF405
Alexa Fluor 488
NB100-56511AF488
Alexa Fluor 532
NB100-56511AF532
Alexa Fluor 594
NB100-56511AF594
Alexa Fluor 647
NB100-56511AF647
Alexa Fluor 700
NB100-56511AF700
Alexa Fluor 750
NB100-56511AF750
Biotin
NB100-56511B
CoraFluor 1
NB100-56511CL1
DyLight 350
NB100-56511UV
DyLight 405
NB100-56511V
DyLight 488
NB100-56511G
DyLight 550
NB100-56511R
DyLight 594
NB100-56511DL594
DyLight 650
NB100-56511C
DyLight 680
NB100-56511FR
DyLight 755
NB100-56511IR
FITC
NB100-56511F
HRP
NB100-56511H
Janelia Fluor 525
NB100-56511JF525
Janelia Fluor 585
NB100-56511JF585
Janelia Fluor 635
NB100-56511JF635
Janelia Fluor 646
NB100-56511JF646
Janelia Fluor 669
NB100-56511JF669
Unconjugated
NB100-56511
mFluor Violet 450 SE
NB100-56511MFV450
mFluor Violet 500 SE
NB100-56511MFV500
mFluor Violet 610 SE
NB100-56511MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56511
BSA Free
NB100-56511
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MyoD Antibody (5.8A) [Janelia Fluor® 549] Summary

Description
There is considerable literature published using the MyoD, Clone 5.8A antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system.
Immunogen
The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).
Specificity
In Rh-30, a ~45 kDa band should be observed.
Predicted Species
Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MyoD Antibody (5.8A) [Janelia Fluor® 549]

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Background

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MyoD Antibody
(5.8A) [Janelia Fluor ...
NB100-56511JF549
MyoD Antibody (5.8A) [Janelia Fluor® 549]
Species: Hu, Mu, Rt, Po
Applications: WB, Flow, ICC/IF, IHC, IP, KD, KO

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Bioinformatics

Gene Symbol MYOD1