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MyBPC3 Antibody (751631)

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Western blot shows lysates of mouse embryonic heart tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse MyBPC3 Monoclonal Antibody (Catalog # MAB7199) followed by HRP-conjugated Anti-Rat IgG Secondary ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
751631
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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MyBPC3 Antibody (751631) Summary

Immunogen
E. coli-derived recombinant mouse MyBPC3
Pro2-Ser169
Accession # O70468
Specificity
Detects mouse MyBPC3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human MyBPC3 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
MYBPC3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MyBPC3 Antibody (751631)

  • Cardiac MyBP-C
  • CMH4
  • C-protein, cardiac muscle isoform
  • DKFZp779E1762
  • FHC
  • MYBP-C
  • MyBPC3
  • myosin binding protein C, cardiac
  • myosin-binding protein C, cardiac
  • myosin-binding protein C, cardiac-type

Background

MyBPC3 (Myosin-binding protein C-cardiac type) is a 140-150 kDa member of the MyBP family, Ig superfamily of proteins. It is expressed in cardiac muscle, and contributes both to myosin filament structure by interacting with light meromysin, and the regulation of contraction by binding to myosin subfragment-2, which results in a reduction of actomyosin ATPase activity. Mouse MyBPC3 is 1270 amino acids (aa) in length. It contains five consecutive C2-type Ig-like domains (aa 151-767), three FN type III repeats (aa 768-958), and two additional C-terminal Ig-like domains (aa 967-1270). There are at least three utilized phosphorylation sites and one Pro‑rich region (aa 100-150). In human, multiple mutations generate variable-length premature truncated forms of MyBPC3. There are two potential isoform variants. One basically shows a six aa substitution for aa 339-344, while a second contains the same six aa substitution coupled to an eight aa extension at the N-terminus. MyBPC3 is a thick filament-associated protein located in the crossbridge region of cardiac muscle sarcomeres. It is known to be a physiological substrate of cAMP‑dependent protein kinase. MyBPC3 has a role in sarcomeric structure and in the regulation of cardiac muscle contraction. MyBPC3 is released into the coronary effluent during myocardial infarction. Mutations in MYBPC3 are associated with hypertrophic cardiomyopathy. Within aa 2-169, mouse MyBPC3 shares 71% and 85% aa sequence identity with human and rat MyBPC3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MYBPC3
Uniprot