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MuRF1/TRIM63 Antibody

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Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-1193] - (1.25ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

  • Size / Catalog#QtyPrice
    NB100-1193
    0.1 mg
    $399.00
    2-4 Business Days
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MuRF1/TRIM63 Antibody Summary

Immunogen
Peptide with sequence DYKSSLIQDGNPM-C corresponding to N-Terminus according to NP_115977.2.
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM63
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in human skeletal muscle lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2 kDa band according to NP_115977.2. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human skeletal muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
Publications
Read Publications using NB100-1193.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MuRF1/TRIM63 Antibody

  • EC 6.3.2.-
  • IRF
  • IRFMURF2
  • Iris RING finger protein
  • MuRF1
  • MuRF-1
  • muscle specific ring finger protein 2
  • Muscle-specific RING finger protein 1
  • RING finger protein 28FLJ32380
  • RNF28
  • RNF28E3 ubiquitin-protein ligase TRIM63
  • SMRZ
  • SMRZMURF-1
  • Striated muscle RING zinc finger protein
  • TRIM63
  • tripartite motif containing 63
  • tripartite motif-containing 63
  • Tripartite motif-containing protein 63

Background

This protein is a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TRIM63
Entrez
Uniprot