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MST4 Antibody

Images

 
Western Blot: MST4 Antibody [NB100-1004] - Staining of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.5 ug/mL. Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MST4 Antibody [NB100-1004] - Staining of paraffin embedded Human Thymus. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MST4 Antibody Summary

Immunogen
Peptide with sequence KLIEKFQKCSADESP corresponding to C-Terminus according to NP_057626.2, NP_001035918.1, NP_001035917.1.
Specificity
This antibody is expected to recognize all three reported isoforms (NP_057626.2, NP_001035918.1 and NP_001035917.1).
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STK26
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/mL
Application Notes
WB: Approx 50kDa band observed in lysates of Jurkat cell lines (calculated MW of 46.5kDa according to NP_057626.2). IHC: Paraffin embedded Human Thymus.
Publications
Read Publication using NB100-1004.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MST4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • Mammalian STE20-like protein kinase 4
  • mammalian sterile 20-like 4
  • MASK
  • Mst3 and SOK1-related kinase
  • MST-4
  • serine/threonine protein kinase MASK
  • serine/threonine protein kinase MST4
  • Serine/threonine-protein kinase MASK
  • serine/threonine-protein kinase MST4
  • STE20-like kinase MST4

Background

Mst4, a member of the GCK family, is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, IHC

Publications for MST4 Antibody (NB100-1004)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK26
Entrez
Uniprot