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MRG-binding protein Antibody - BSA Free

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Orthogonal Strategies: Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Analysis in human testis and liver tissues using NBP1-84936 antibody. Corresponding MRGBP RNA-seq data are ...read more
Western Blot: MRG-binding protein Antibody [NBP1-84936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-352
Immunocytochemistry/ Immunofluorescence: MRG-binding protein Antibody [NBP1-84936] - Staining of human cell line U-2 OS shows localization to nucleus.
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Skeletal muscle shows very weak nuclear positivity in myocytes.
Western Blot: MRG-binding protein Antibody [NBP1-84936] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Pancreas shows very weak nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: MRG-binding protein Antibody [NBP1-84936] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
       

Orthogonal Strategies

 

Order Details

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Catalog# & Formulation Size Price

MRG-binding protein Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LFHAMLGHKPVGVNRHFHMICIRDKFSQNIGRQVPSKVIWDHLSTMYDMQALHESEILPFPNPERNFVLPEEIIQEVREGKVMIEEEMKEEMKEDVDPHNGADDVFSSSGSLGKASEKSSKDKEKNSSDLGCKE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MRGBP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MRG-binding protein Protein (NBP1-84936PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MRG-binding protein Antibody - BSA Free

  • chromosome 20 open reading frame 20
  • Eaf7
  • FLJ10914
  • MRG(MORF4-related gene)-binding protein
  • MRG15BP
  • MRG-binding protein
  • MRGBPURCC4

Background

C20orf20 is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MRGBP