![Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining in human epididymis and skeletal muscle tissues using anti-CUL4B antibody. Corresponding CUL4B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0015.jpg "Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining in human epididymis and skeletal muscle tissues using anti-CUL4B antibody. Corresponding CUL4B RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: GDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CUL4B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Theoretical MW | 104 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-86099 | Applications | Species |
|---|---|---|
| Li T, Wu S, Jia L et al. CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma Cancer Biol Med 2020-05-15 [PMID: 32587774] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for CUL4B Antibody (NBP1-86099)Find related products by research area.
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![Western Blot: CUL4B Antibody [NBP1-86099] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Western-Blot-NBP1-86099-img0008.jpg "Western Blot: CUL4B Antibody [NBP1-86099] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: CUL4B Antibody [NBP1-86099] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86099-img0009.jpg "Immunocytochemistry/Immunofluorescence: CUL4B Antibody [NBP1-86099] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0014.jpg "Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human skeletal muscle shows low expression as expected.")
![Western Blot: CUL4B Antibody [NBP1-86099] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Western-Blot-NBP1-86099-img0007.jpg "Western Blot: CUL4B Antibody [NBP1-86099] - Analysis in human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0013.jpg "Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human epididymis shows high expression.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")