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Mouse IFN-gamma Quantikine ELISA Kit, 2 Plate

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse IFN-gamma Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-gamma in cell culture supernates and mouse serum. It contains E. coli-expressed mouse IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IFN-gamma accurately. Results obtained using natural mouse IFN-gam...ma showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-gamma.
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Specificity
Natural and recombinant mouse IFN-gamma
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Ifng

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MIF00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IFN-gamma Quantikine ELISA Kit, 2 Plate

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IFN-gamma (MIF00)(306)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.


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Human
(2)
Mouse
(300)
Rat
(2)
Transgenic Mouse
(4)
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Showing Publications 1 - 10 of 306. Show All 306 Publications.
Publications using MIF00 Applications Species
Shin, SH;Ye, MK;Chae, MH;Geum, SY;Aboraia, AS;Abdel-Aal, AM;Qayed, WS;El-Wahab, HAAA;Abou-Ghadir, OF;Aboul-Fadl, T; Anti-Allergic and Anti-Inflammatory Effects of Lidocaine-Derived Organic Compounds in a House Dust Mite-Induced Allergic Rhinitis Mouse Model Biomedicines 2024-08-29 [PMID: 39335479] (Mouse) Mouse
Taheri, M;Tehrani, HA;Farzad, SA;Korourian, A;Arefian, E;Ramezani, M; The potential of mesenchymal stem cell coexpressing cytosine deaminase and secretory IL18-FC chimeric cytokine in suppressing glioblastoma recurrence International immunopharmacology 2024-09-04 [PMID: 39236459] (Mouse) Mouse
Blanco, T;Nakagawa, H;Musayeva, A;Krauthammer, M;Singh, RB;Narimatsu, A;Ge, H;Shoushtari, SI;Dana, R; Acquired Immunostimulatory Phenotype of Migratory CD103+ Dendritic Cells Promotes Alloimmunity Following Corneal Transplantation JCI insight 2024-09-05 [PMID: 39235864] (Mouse) Mouse
Walter Jackson Iii, ;Yang, Y;Salman, S;Dordai, D;Lyu, Y;Datan, E;Drehmer, D;Huang, TY;Hwang, Y;Semenza, GL; Pharmacologic HIF stabilization activates costimulatory receptor expression to increase antitumor efficacy of adoptive T cell therapy Science advances 2024-08-30 [PMID: 39196939] (Transgenic Mouse) Transgenic Mouse
Tripathi, A;Bartosh, A;Mata, J;Jacks, C;Madeshiya, AK;Hussein, U;Hong, LE;Zhao, Z;Pillai, A; Microglial type I interferon signaling mediates chronic stress-induced synapse loss and social behavior deficits Molecular psychiatry 2024-08-02 [PMID: 39095477] (Mouse) Mouse
Zhang, Y;Lee, C;Geng, S;Wang, J;Bohara, U;Hou, J;Yi, Z;Li, L; Immune-enhancing neutrophils reprogrammed by subclinical low-dose endotoxin in cancer treatment EMBO molecular medicine 2024-07-15 [PMID: 39009886] (Human) Human
Liu, J;Huang, B;Rao, Y;Guo, L;Cai, C;Gao, D;Kong, D;Wang, G;Xiong, Y;Cui, R;Zhang, M;Chen, C; Intraductal photothermal ablation: a noninvasive approach for early breast cancer treatment and prevention Theranostics 2024-07-01 [PMID: 38994019] (Mouse) Mouse
Zhou, B;Elean, M;Arce, L;Fukuyama, K;Tomotsune, K;Dentice Maidana, S;Saha, S;Namai, F;Nishiyama, K;Vizoso-Pinto, MG;Villena, J;Kitazawa, H; The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract Microorganisms 2024-06-16 [PMID: 38930591] (Mouse) Mouse
Li, Z;Wang, S;Han, J;Yang, G;Xi, L;Zhang, C;Cui, Y;Yin, S;Zhang, Y;Zhang, H; Insights into the effect of guanylate-binding protein 1 on the survival of Brucella intracellularly Veterinary microbiology 2024-04-16 [PMID: 38678845] (Mouse) Mouse
Wang, Z;Chen, Y;Wu, H;Wang, M;Mao, L;Guo, X;Zhu, J;Ye, Z;Luo, X;Yang, X;Liu, X;Yang, J;Sheng, Z;Lee, J;Guo, Z;Liu, Y; Intravenous administration of IL-12 encoding self-replicating RNA-lipid nanoparticle complex leads to safe and effective antitumor responses Scientific reports 2024-03-28 [PMID: 38548896] (Mouse) Mouse
Show All 306 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IFN-gamma (MIF00). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Additional IFN-gamma Products

Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Bioinformatics

Gene Symbol Ifng