![Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Western-Blot-NBP3-15411-img0004.jpg "Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, IHC |
| Clone | 5M8A5 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Monoamine Oxidase B (MAOB) (P27338). MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFR |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MAOB |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Monoamine Oxidase B Antibody (5M8A5) validated for Simple Western from a verified customer review. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Stella Dracheva |
Simple Western | Human | 04/06/2023 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Monoamine Oxidase B Antibody (NBP3-15411)Find related products by research area.
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| Stella Dracheva 04/06/2023 |
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| Application: | Simple Western | |
| Species: | Human |
| Gene Symbol | MAOB |
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Mouse testis using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Immunohistochemistry-Paraffin-NBP3-15411-img0003.jpg "Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Mouse testis using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Rat lung using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Immunohistochemistry-Paraffin-NBP3-15411-img0001.jpg "Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Rat lung using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Human colon using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Immunohistochemistry-Paraffin-NBP3-15411-img0002.jpg "Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Human colon using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Simple Western: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Monoamine Oxidase B Antibodies (NBP3-15411), ProteinSimple Western Blot on Jess Instrument. 1 microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-15411_rabbit-monoamine-oxidase-b-mab-5m8a5-simple-western-2042023112630.jpg "Simple Western: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Monoamine Oxidase B Antibodies (NBP3-15411), ProteinSimple Western Blot on Jess Instrument. 1 microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
