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MMP-8 Antibody [Unconjugated]

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Western blot shows lysates of rat lung tissue and rat spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-8 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of rat lung tissue and rat spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3245) followed by ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3245-SP
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MMP-8 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MMP-8
Leu21-Pro466
Accession # O88766
Specificity
Detects rat and mouse MMP-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human MMP‑8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
AF3245 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-8 Antibody [Unconjugated]

  • CLG1HNC
  • Collagenase 2
  • EC 3.4.24
  • EC 3.4.24.34
  • matrix metallopeptidase 8 (neutrophil collagenase)
  • matrix metalloproteinase 8 (neutrophil collagenase)
  • Matrix metalloproteinase-8
  • MMP8
  • MMP-8
  • neutrophil collagenase
  • PMNL collagenase
  • PMNL-CL

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase, collagenase 2) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II, and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein, and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP-8 has a high capacity for infiltrating connective tissue and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region, and a hemopexin-like domain (1).

  1. Tschesche, T. et al. (2004) Handbook of Proteolytic Enzymes (eds. A.J. Barrett et al.) p.480, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-8 Antibody (AF3245)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Neutralization.


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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MMP8
Uniprot