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MMP-7 Antibody (377313) - Pro form

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Western blot shows lysates of NS0 mouse myeloma cell line transfected with with mouse MMP-7. PVDF Membrane was probed with 2 µg/mL of Mouse Pro-MMP-7 Monoclonal Antibody (Catalog # MAB29671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, active rmMMP-7 was derived from the proteolytic activation of Recombinant Mouse MMP-7 (Catalog # 2967-MP) (20 ng/lane) and probed with 1 µg/mL of Mouse MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967). Specific bands were detected for Pro-MMP-7 at approximately 35 kDa and active MMP-7 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NS0 mouse myeloma cell line transfected with with mouse MMP-7. PVDF Membrane was probed with 2 µg/mL of Mouse Pro-MMP-7 Monoclonal Antibody (Catalog # MAB29671) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
377313
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB29671-SP
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MMP-7 Antibody (377313) - Pro form Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP‑7
Leu18-Leu264
Accession # AAA99983
Specificity
Detects the pro form of mouse MMP-7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse MMP‑2, -3, -8, -9, -12, or -24 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
MMP7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB29671 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-7 Antibody (377313) - Pro form

  • EC 3.4.24
  • EC 3.4.24.23
  • Matrilysin
  • matrin
  • matrix metallopeptidase 7 (matrilysin, uterine)
  • matrix metalloproteinase 7 (matrilysin, uterine)
  • Matrix metalloproteinase-7
  • MMP7
  • MMP-7
  • MPSL1
  • Pump-1 protease
  • PUMP1
  • PUMP-1
  • uterine matrilysin
  • Uterine metalloproteinase

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin, and versican (1). MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

  1. Woessner, J.F. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-7 Antibody (MAB29671)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol MMP7
Uniprot