MMP-12 ELISA Development Kit

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is critical for invasion and destruction in pathologies such as aneurysm and emphysema. HME mRNA and protein were previously described as expressed in human alveolar macrophages (Curci JA et al, 1998, J Clin Invest, 102(11):1900-10). The predicted molecular mass of the HME proenzyme is 54 kDa and readily undergoes NH2- and COOH-terminal processing to an active 45KDa form and a mature 22 kDa form. HME is a unique human metalloproteinase that displays elastolytic activity. We have isolated HME-MMP12 cDNA from a human CD34+-derived DC (GM-CSF+TNF) library. Expression analysis revealed HME-MMP12 expression in lung and placenta. Anti-HME-MMP12 monoclonal antibodies were obtained after mice immunization with HME-MMP12-transfected eukaryotic cells. These antibodies were used to set up a quantitative immunoassay for the detection of human HME-MMP12 (Demedts IK et al, 2006; Thorax, 61:196-201; Lapan et al, BMC Pulmonary Medicine, 2010, 10:40).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Showing Publications 1 - 2 of 2.

Publications using DDXK-E-MMP12-1	Applications	Species
Lapan P et al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med 2010-08-02 [PMID: 20678199]
Demets IK et al. Elevated MMP-12 protein levels in induced sputum from patients with COPD. Thorax 2006-03-01 [PMID: 16308335]

Array DDXK-E-MMP12-1

• MMP-12 Antibodies
• MMP-12 ELISA Kits
• MMP-12 Kit
• MMP-12 Proteins and Enzymes