MKP-1/DUSP1 Antibody

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publication using NB100-834	Applications	Species
Alessi DR, Smythe C, Keyse SM. The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts. Oncogene 1993-07-01 [PMID: 8390041]

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Alexander Vang	WB	Rat	03/19/2018	View Summary Application Western Blot Sample Tested Heart lysates Species Rat Lot S2 E190417 Comments Comments Proteins were separated on SDS-PAGE and transferred onto PVDF membrane. Membrane was blocked with 5% milk in TBS 0.01% tween and then incubated with primary antibody overnight 1:1000. Secondary was used at 1:5000 and then membrane was ECL and imaged with GE Imagequant imager.

Array NB100-834

• MKP-1/DUSP1 Antibodies
• MKP-1/DUSP1 ELISA Kits
• MKP-1/DUSP1 Proteins