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MKK6/MEK6 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining in human colon and testis tissues using anti-MAP2K6 antibody. Corresponding MAP2K6 RNA-seq data are presented for ...read more
Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human cell line A549 shows localization to nucleus & cytosol.
Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-341
Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human colon shows high expression.
Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human testis shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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MKK6/MEK6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP2K6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MKK6/MEK6 Protein (NBP1-87791PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MKK6/MEK6 Antibody

  • MAP kinase kinase 6
  • MAP2K6
  • MAPK/ERK kinase 6
  • MAPKK6dual specificity mitogen-activated protein kinase kinase 6
  • MEK6
  • MEK6MAPKK 6
  • mitogen-activated protein kinase kinase 6
  • MKK6
  • MKK6MEK 6
  • PRKMK6
  • PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
  • SAPKK3
  • SAPKK3EC 2.7.12.2

Background

Mitogen-acitvated protein kinase kinase 6 (MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK6, closely related to MKK3, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MTK1 phosphorylates MKK6 on serine 207 and threonine 211 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAP2K6