Reactivity | RtSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 120 amino acids corresponding to Midkine Source: E. coli Uniprot ID: Q9R1S9 Amino Acid Sequence: VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD |
Details of Functionality | Midkine Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | MDK |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note | Less than 0.1 EU/ug of Midkine as determined by LAL method. |
Dilutions |
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Theoretical MW | 13.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. |
Research Areas for Midkine Protein (NBP2-35273)Find related products by research area.
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Gene Symbol | MDK |