MFG-E8 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C-terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Mouse, Bovine, Transgenic Mouse.

We have publications tested in 4 applications: IHC, Immunoprecipitation, In Vivo, Western Blot.

Showing Publications 1 - 10 of 24. Show All 24 Publications. Collapse Publications.

Publications using AF2805	Applications	Species
Sokolova, D;Ghansah, SA;Puletti, F;Georgiades, T;De Schepper, S;Zheng, Y;Crowley, G;Wu, L;Rueda-Carrasco, J;Koutsiouroumpa, A;Muckett, P;Freeman, OJ;Khakh, BS;Hong, S; Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models bioRxiv : the preprint server for biology 2024-09-01 [PMID: 39257734] (Western Blot, Transgenic Mouse)	Western Blot	Transgenic Mouse
Y Itoh, V Sahni, SJ Shnider, H McKee, JD Macklis Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations Cell Reports, 2023-03-17;42(3):112182. 2023-03-17 [PMID: 36934325] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
D Han, D Lu, S Huang, J Pang, Y Wu, J Hu, X Zhang, Y Pi, G Zhang, J Wang Small extracellular vesicles from Ptpn1-deficient macrophages alleviate intestinal inflammation by reprogramming macrophage polarization via lactadherin enrichment Redox Biology, 2022-11-28;58(0):102558. 2022-11-28 [PMID: 36462232] (In Vivo, Transgenic Mouse)	In Vivo	Transgenic Mouse
J Wagner, K Degenhardt, M Veit, N Louros, K Konstantou, A Skodras, K Wild, P Liu, U Obermüller, V Bansal, A Dalmia, LM Häsler, M Lambert, M De Vleesch, HA Davies, J Madine, D Kronenberg, R Feederle, D Del Turco, KPR Nilsson, T Lashley, T Deller, M Gearing, LC Walker, P Heutink, F Rousseau, J Schymkowit, M Jucker, JJ Neher Medin co-aggregates with vascular amyloid-beta in Alzheimer's disease Nature, 2022-11-16;0(0):. 2022-11-16 [PMID: 36385530] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
Minmin Cai, Liufang Sheng MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways Stem Cells International 2022-10-12 [PMID: 36277041]
Leng Ni, Lijuan Liu, Wanqu Zhu, Richard Telljohann, Jing Zhang, Robert E. Monticone et al. Inflammatory Role of Milk Fat Globule–Epidermal Growth Factor VIII in Age‐Associated Arterial Remodeling Journal of the American Heart Association 2022-09-06 [PMID: 36000422]
G Han, H Kim, DE Kim, Y Ahn, J Kim, YJ Jang, K Kim, Y Yang, SH Kim The Potential of Bovine Colostrum-Derived Exosomes to Repair Aged and Damaged Skin Cells Pharmaceutics, 2022-01-27;14(2):. 2022-01-27 [PMID: 35214040] (Western Blot, Bovine)	Western Blot	Bovine
P Marazuela, M Solé, A Bonaterra-, J Pizarro, J Camacho, E Martínez-S, HB Kuiperij, MM Verbeek, AM de Kort, FHBM Schreuder, CJM Klijn, L Castillo-R, O Pancorbo, D Rodríguez-, F Pujadas, P Delgado, M Hernández- MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy Acta neuropathologica communications, 2021-09-16;9(1):154. 2021-09-16 [PMID: 34530925] (IHC, Western Blot, Mouse, Transgenic Mouse)	IHC, Western Blot	Mouse, Transgenic Mouse
Y Lu, L Liu, J Pan, B Luo, H Zeng, Y Shao, H Zhang, H Guan, D Guo, C Zeng, R Zhang, X Bai, H Zhang, D Cai MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-&kappaB pathway Cell Death & Disease, 2021-05-25;12(6):533. 2021-05-25 [PMID: 34031369] (Western Blot, Mouse)	Western Blot	Mouse
HF Bu, S Subramania, H Geng, X Wang, F Liu, PM Chou, C Du, IG De Plaen, XD Tan MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice Cells, 2021-03-25;10(4):. 2021-03-25 [PMID: 33806041] (Western Blot, Mouse)	Western Blot	Mouse
C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain Nature Communications, 2020-11-30;11(1):6133. 2020-11-30 [PMID: 33257673] (Western Blot, Mouse)	Western Blot	Mouse
Lander Egaña-Gorroño, Prameladevi Chinnasamy, Isabel Casimiro, Vanessa M. Almonte, Dippal Parikh, Gustavo H. Oliveira-Paula et al. Allograft inflammatory factor-1 supports macrophage survival and efferocytosis and limits necrosis in atherosclerotic plaques Atherosclerosis 10/1/2019 [PMID: 31439353]
S Eguchi, M Takefuji, T Sakaguchi, S Ishihama, Y Mori, T Tsuda, T Takikawa, T Yoshida, K Ohashi, Y Shimizu, R Hayashida, K Kondo, YK Bando, N Ouchi, T Murohara Cardiomyocytes capture stem cell-derived, anti-apoptotic microRNA-214 via clathrin-mediated endocytosis in acute myocardial infarction J. Biol. Chem., 2019-06-19;0(0):. 2019-06-19 [PMID: 31217281] (Western Blot, Mouse)	Western Blot	Mouse
Stefan Christopher Weise, Alejandro Villarreal, Stefanie Heidrich, Fariba Dehghanian, Christian Schachtrup, Sigrun Nestel et al. TGF beta -Signaling and FOXG1-Expression Are a Hallmark of Astrocyte Lineage Diversity in the Murine Ventral and Dorsal Forebrain Frontiers in Cellular Neuroscience 2018-11-28 [PMID: 30555301]
J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia Cell Rep, 2018-08-21;24(8):1939-1948.e4. 2018-08-21 [PMID: 30134156] (Western Blot, Mouse)	Western Blot	Mouse
CA Reardon, A Lingaraju, KQ Schoenfelt, G Zhou, C Cui, H Jacobs-El, I Babenko, A Hoofnagle, D Czyz, H Shuman, T Vaisar, L Becker Obesity and Insulin Resistance Promote Atherosclerosis through an IFN?-Regulated Macrophage Protein Network Cell Rep, 2018-06-05;23(10):3021-3030. 2018-06-05 [PMID: 29874587] (Western Blot, Mouse)	Western Blot	Mouse
Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release JCI Insight, 2018-04-19;3(8):. 2018-04-19 [PMID: 29669945] (Western Blot, Mouse)	Western Blot	Mouse
JH Wang, AV Forterre, J Zhao, DO Frimannsso, A Delcayre, TJ Antes, B Efron, SS Jeffrey, MD Pegram, AC Matin Anti-HER2 scFv-directed extracellular vesicle-mediated mRNA-based gene delivery inhibits growth of HER2-positive human breast tumor xenografts by prodrug activation Mol. Cancer Ther., 2018-02-26;0(0):. 2018-02-26 [PMID: 29483213] (Western Blot, Mouse)	Western Blot	Mouse
SA Yuzwa, MJ Borrett, BT Innes, A Voronova, T Ketela, DR Kaplan, GD Bader, FD Miller Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling Cell Rep, 2017-12-26;21(13):3970-3986. 2017-12-26 [PMID: 29281841] (IHC, Mouse)	IHC	Mouse
Saewu A, Kadunganattil S, Raghupathy R et al. Clusterin in the mouse epididymis: possible roles in sperm maturation and capacitation. Reproduction. 10/2/2017 [PMID: 28971894]
Spherical nucleic acid targeting microRNA-99b enhances intestinal MFG-E8 gene expression and restores enterocyte migration in lipopolysaccharide-induced septic mice Sci Rep, 2016-08-19;6(0):31687. 2016-08-19 [PMID: 27538453] (Western Blot, Mouse)	Western Blot	Mouse
Xiao Wang, Heng-Fu Bu, Shirley X L Liu, Isabelle G. De Plaen, Xiao-Di Tan Molecular Mechanisms Underlying the Regulation of the MFG-E8 Gene Promoter Activity in Physiological and Inflammatory Conditions Journal of Cellular Biochemistry 2015-09-01 [PMID: 25711369]
Khalifeh-Soltani A, McKleroy W, Sakuma S et al. Mfge8 promotes obesity by mediating the uptake of dietary fats and serum fatty acids. Nat. Med. 2/7/2014 [PMID: 24441829]
Fuller AD, Van Eldik LJ MFG-E8 regulates microglial phagocytosis of apoptotic neurons. J Neuroimmune Pharmacol, 2008-08-01;3(4):246-56. 2008-08-01 [PMID: 18670887] (Western Blot, Mouse)	Western Blot	Mouse
Show All 24 Publications. Collapse Publications.

Array AF2805

• MFG-E8 Antibodies
• MFG-E8 Lysate
• MFG-E8 Protein
• MFG-E8 Proteins and Enzymes
• MFG-E8 ELISAs