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Melanocortin-3 R/MC3R Antibody

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Western Blot: Melanocortin-3 R/MC3R Antibody [NB100-1501] - (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: Melanocortin-3 R/MC3R Antibody [NB100-1501] - (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Melanocortin-3 R/MC3R Antibody Summary

Immunogen
Peptide with sequence SIQKTYLEGDFV-C corresponding to N-Terminus according to NP_063941.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MC3R
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in human brain lysates (calculated MW of 40.1 kDa band according to NP_063941.2).
Reviewed Applications
Read 1 Review rated 4
using
NB100-1501 in the following applications:
Publications
Read Publication using NB100-1501.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Melanocortin-3 R/MC3R Antibody

  • BMIQ9
  • MC3
  • MC3R
  • MC3-R
  • melanocortin 3 receptor
  • melanocortin receptor 3
  • Melanocortin-3 R
  • Melanocortin3R
  • Melanocortin-3R
  • OB20
  • Obesity quantitative trait locus
  • OQTL

Background

Melanocortins are ligands for a subgroup of GPCRs positively coupled to Adenylate cylcases, termed as melanocortin receptors (MC1R, MC2R, MC3R, MC4R amd MC5R). These receptors are widely expressed at varied density throughout the body. The MC3R has been widely implicated in inflammation and is also implicated in a role in obesity and metabolism. MC3R KO mice exhibit reduced linear growth and lean mass and are hyperleptinemic and hyperinsulinemic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melanocortin-3 R/MC3R Antibody (NB100-1501)(1)


Review for Melanocortin-3 R/MC3R Antibody (NB100-1501) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1501:
Filter by Applications
Flow
(1)
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(1)
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Images Ratings Applications Species Date Details
Flow Cytometry Melanocortin-3 R/MC3R NB100-1501
Enlarge 		4
reviewed by:
Quentin Howlett-Prieto
 Flow Human 02/05/2019
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Summary

ApplicationFlow Cytometry
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesHuman
LotP1

Comments

CommentsBlocking: Natural Mouse Serum
Primary antibody: 4 uL (2 ug)
Secondary antibody: Alexa Fluor 488 Rabbit anti-Goat IgG (2.5 uL / .5 ug)

Details: Human lymphocytes were isolated from PBMCs. Lymphocytes were blocked with Natural Mouse Serum for 15 minutes, and then stained and subsequently incubated at -20 C with anti-human MC3R primary antibody for 30 minutes. After washing, cells were stained with Alexa Fluor 488 Rabbit anti-Goat IgG and incubated at -20 C for 40 minutes. After washing, cells were detected using a Fortessa 4-15 flow cytometer.

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Secondary Antibodies

 

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Recent Reviews

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1
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Flow Cytometry Melanocortin-3 R/MC3R NB100-1501
Enlarge
 Quentin Howlett-Prieto
02/05/2019
Application: Flow
Species: Human
read this review

Bioinformatics

Gene Symbol MC3R
Entrez
Uniprot