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MEKK2 Antibody

Images

 
Western Blot: MEKK2 Antibody [NBP2-48554] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: MEKK2 Antibody [NBP2-48554] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: MEKK2 Antibody [NBP2-48554] - Staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MEKK2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: IAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQM
Predicted Species
Mouse (91%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MEKK2 Recombinant Protein Antigen (NBP2-48554PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MEKK2 Antibody

  • EC 2.7.11.25
  • MAP/ERK kinase kinase 2
  • MAP3K2
  • MAPK/ERK kinase kinase 2
  • MAPKKK2
  • MEK kinase 2
  • MEKK 2
  • MEKK2
  • MEKK2B
  • mitogen-activated protein kinase kinase kinase 2

Background

MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP3K2