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MEKK2 Antibody (SR3035)

Images

 
Western Blot: MEKK2 Antibody (SR3035) [NBP3-27740] - Western blot analysis of MEKK2 expression in HeLa cell lysate.
Immunohistochemistry-Paraffin: MEKK2 Antibody (SR3035) [NBP3-27740] - Immunohistochemical analysis of paraffin-embedded human thyroid cancer

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
SR3035
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MEKK2 Antibody (SR3035) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human MEKK2 (Uniprot #: Q9Y2U5)
Specificity
Detects endogenous levels of MEKK2
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MAP3K2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:30
  • Western Blot 1:5000-1:20000
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 150mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MEKK2 Antibody (SR3035)

  • EC 2.7.11.25
  • MAP/ERK kinase kinase 2
  • MAP3K2
  • MAPK/ERK kinase kinase 2
  • MAPKKK2
  • MEK kinase 2
  • MEKK 2
  • MEKK2
  • MEKK2B
  • mitogen-activated protein kinase kinase kinase 2

Background

MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MEKK2 Antibody (NBP3-27740) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP3K2