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MEF2C Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Analysis of human cerebral cortex and liver tissues. Corresponding MEF2C RNA-seg data are presented for the same tissues.
Western Blot: MEF2C Antibody [NBP1-89468] - Analysis in control (vector only transfected HEK293T lysate) and MEF2C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP1-89468] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human liver shows no postivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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MEF2C Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINN
Predicted Species
Mouse (97%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MEF2C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MEF2C Protein (NBP1-89468PEP)
Publications
Read Publications using NBP1-89468.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MEF2C Antibody

  • DEL5q14.3
  • MEF2C
  • myocyte enhancer factor 2C
  • myocyte-specific enhancer factor 2C
  • polypeptide C

Background

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture . Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MEF2C Antibody (NBP1-89468) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on MEF2C

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Bioinformatics

Gene Symbol MEF2C