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MDH2 Antibody

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Independent Antibodies: Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-89540 (A) shows similar protein distribution ...read more
Genetic Strategies: Western Blot: MDH2 Antibody [NBP1-89540] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: MDH2 Antibody [NBP1-89540] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining shown in green.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Western Blot: MDH2 Antibody [NBP1-89540] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human colon.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human lymph node.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human kidney.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human pancreas shows moderate to strong granular cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human skeletal muscle shows moderate to strong granular cytoplasmic positivity in myocytes
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human liver shows moderate to strong granular cytoplasmic positivity in hepatocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MDH2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
Control Peptide
MDH2 Protein (NBP1-89540PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MDH2 Antibody

  • EC 1.1.1
  • EC 1.1.1.37
  • malate dehydrogenase 2, NAD (mitochondrial)
  • MDH
  • MDH2
  • MGC:3559
  • mitochondrial
  • MOR1

Background

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MDH2