Genetic Strategies: Western Blot: MDH2 Antibody [NBP1-86036] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: MDH2 Antibody [NBP1-86036] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human colon.
Western Blot: MDH2 Antibody [NBP1-86036] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human lymph node.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human liver.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human kidney.
Independent Antibodies: Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-86036 (A) shows similar protein distribution ...read more
This antibody was developed against Recombinant Protein corresponding to amino acids: VTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDH2
Purity
Immunogen affinity purified
Innovator's Reward
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WB reported in scientific literature (PMID: 25763823). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reactivity reported in scientific literature (PMID: 23334578)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for MDH2 Antibody
EC 1.1.1
EC 1.1.1.37
malate dehydrogenase 2, NAD (mitochondrial)
MDH
MDH2
MGC:3559
mitochondrial
MOR1
Background
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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