![Western Blot: MDH1 Antibody [HRP] [NB100-2019] - Western Blot of Sheep MDH1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: MDH1 [0.05ug]. Primary Antibody: Anti-MDH1 HRP at 1:1000 for 1 hr at RT. Secondary Antibody: none. Blocking Buffer for 30mins at RT. Predicted MW: 30-35kDa. Exposure: 1sec.](http://images.novusbio.com/fullsize/MDH1-Antibody-[HRP]-Western-Blot-NB100-2019-img0001.jpg "Western Blot: MDH1 Antibody [HRP] [NB100-2019] - Western Blot of Sheep MDH1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: MDH1 [0.05ug]. Primary Antibody: Anti-MDH1 HRP at 1:1000 for 1 hr at RT. Secondary Antibody: none. Blocking Buffer for 30mins at RT. Predicted MW: 30-35kDa. Exposure: 1sec.")
| Reactivity | PoSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | HRP |
| Concentration | LYOPH |
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart] |
| Immunogen | MDH1 [Pig Heart] (Uniprot: P11708) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | MDH1 |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
| Readout System |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MDH1 Antibody (NB100-2019)Find related products by research area.
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