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MDA5 Antibody (1B6)

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Western Blot: MDA5 Antibody (1B6) [NBP1-97526] - Analysis of MDA5 monoclonal antibody (1B6) : Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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MDA5 Antibody (1B6) Summary

Immunogen
Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IFIH1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for MDA5 Antibody (1B6)

  • AGS7
  • CADM-140 autoantigen
  • Clinically amyopathic dermatomyositis autoantigen 140 kDa
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • EC 3.6.4.13
  • Helicard
  • Helicase with 2 CARD domains
  • Hlcd
  • IDDM19
  • IFIH1
  • IMD95
  • interferon induced with helicase C domain 1
  • interferon-induced helicase C domain-containing protein 1
  • Interferon-induced with helicase C domain protein 1
  • MDA5
  • MDA-5
  • MDA-5MGC133047
  • MDA5RNA helicase-DEAD box protein 116
  • melanoma differentiation associated protein-5
  • Melanoma differentiation-associated protein 5
  • Murabutide down-regulated protein
  • RH116
  • SGMRT1

Background

MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow easier access of CAD, thereby accelerating the degradation of genomic DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MDA5 Antibody (NBP1-97526) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for MDA5 Antibody (NBP1-97526)

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Blogs on MDA5.


  Read full blog post.

MDA5 - Part of the RIG-I-like Receptor Family
The innate immune system is responsible for reacting to viral infections through recognition of various viral components. Like toll-like receptor 3 (TLR3), MDA5 recognizes double-stranded (ds) RNA which is a molecular pattern indicative of viral infec...  Read full blog post.

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Bioinformatics

Gene Symbol IFIH1