MDA5 Antibodies
We offer MDA5 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each MDA5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MDA5 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the MDA5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MDA5 polyclonal antibodies and browse our MDA5 monoclonal antibody catalog.
Alternate Names for MDA5 Antibodies
anti-MDA5 antibody, anti-IFIH1 antibody, anti-CADM-140 autoantigen antibody, anti-Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody, anti-DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide antibody, anti-EC 3.6.4.13 antibody, anti-helicard antibody, anti-Helicase with 2 CARD domains antibody, anti-Hlcd antibody, anti-IDDM19 antibody, anti-interferon induced with helicase C domain 1 antibody, anti-interferon-induced helicase C domain-containing protein 1 antibody, anti-Interferon-induced with helicase C domain protein 1 antibody, anti-MDA-5MGC133047 antibody, anti-MDA5RNA helicase-DEAD box protein 116 antibody, anti-melanoma differentiation associated protein-5 antibody, anti-Melanoma differentiation-associated protein 5 antibody, anti-Murabutide down-regulated protein antibody, anti-RH116 antibody
14 Results for "mda5" in Primary Antibodies
![Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NB110-1244-img0001.jpg "Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Flow Cytometry: MDA5 Antibody [NB110-1244] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MDA5-Antibody-Flow-Cytometry-NB110-1244-img0002.jpg)
![Western Blot: MDA5 Antibody [NBP1-76760] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/MDA5-Antibody-Western-Blot-NBP1-76760-img0001.jpg "Western Blot: MDA5 Antibody [NBP1-76760] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunocytochemistry/Immunofluorescence: MDA5 Antibody [NBP1-76760] - Human Lymph Node cells.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76760-img0003.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76760] - Analysis of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-76760-img0004.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76760] - Human lymph node tissue with MDA5 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-76760-img0002.jpg)
![Immunohistochemistry: MDA5 Antibody - BSA Free [NBP1-76760] - Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76760_rabbit-polyclonal-mda5-antibody-2711202419553092.gif)
![Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76760] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76760_rabbit-polyclonal-mda5-antibody-271120241945129.jpg)
![Immunohistochemistry: MDA5 Antibody - BSA Free [NBP1-76760] - Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76760_rabbit-polyclonal-mda5-antibody-2711202420285847.gif)
![Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76760] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76760_rabbit-polyclonal-mda5-antibody-271120241943222.jpg)
![Western Blot: MDA5 Antibody [NBP1-76761] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/MDA5-Antibody-Western-Blot-NBP1-76761-img0001.jpg "Western Blot: MDA5 Antibody [NBP1-76761] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunocytochemistry/Immunofluorescence: MDA5 Antibody [NBP1-76761] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76761-img0003.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76761] - Human lymph node tissue with MDA5 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-76761-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76761] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76761_rabbit-polyclonal-mda5-antibody-2711202420333830.jpg)
![Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76761] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76761_rabbit-polyclonal-mda5-antibody-2711202420212315.jpg)
![Western Blot: MDA5 Antibody [NBP1-89685] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: SK-MEL-30](http://images.novusbio.com/fullsize/MDA5-Antibody-Western-Blot-NBP1-89685-img0005.jpg "Western Blot: MDA5 Antibody [NBP1-89685] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: SK-MEL-30")
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-89685] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89685-img0004.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243] - Human breast carcinoma.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP2-17243-img0002.jpg "Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243] - Human breast carcinoma.")
![<b>Biological Strategies Validation. </b>Western Blot: MDA5 Antibody [NBP2-17243] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDA5 antibody [N2C1], Internal (NBP2-17243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17243_rabbit-polyclonal-mda5-antibody-910202420134057.jpg)
![Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MDA5-Antibody-2N8Z5-Western-Blot-NBP3-16132-img0001.jpg "Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of BxPC-3 cells, using MDA5 antibody (NBP3-16132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MDA5-Antibody-2N8Z5-Western-Blot-NBP3-16132-img0002.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody (3F6) [H00064135-M02] - Analysis of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-3F6-Immunohistochemistry-Paraffin-H00064135-M02-img0002.jpg "Immunohistochemistry-Paraffin: MDA5 Antibody (3F6) [H00064135-M02] - Analysis of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: MDA5 Antibody (3F6) [H00064135-M02] - Detection limit for recombinant GST tagged IFIH1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDA5-Antibody-3F6-Sandwich-ELISA-H00064135-M02-img0003.jpg)
![Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] - Western blot analysis using NBP3-21840 in THP1 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21840_rabbit-mda5-mab-sr1076-57202316112145.jpg "Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] - Western blot analysis using NBP3-21840 in THP1 cell lysate.")
![Western Blot: MDA5 Antibody (PSH03-56) [NBP3-32581] - Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (NBP3-32581) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Daudi cell lysate (20 ug/Lane) Lane 5: Raji cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: HeLa cell lysate (20 ug/Lane) Lane 8: SH-SY5Y cell lysate (20 ug/Lane) Lane 9: A549 cell lysate (20 ug/Lane) Lane 10: A431 cell lysate (20 ug/Lane) Lane 11: Mouse pancreas tissue lysate (40 ug/Lane) Lane 12: Rat kidney tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32581) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32581_rabbit-mda5-mab-psh03-56-278202415295575.jpg "Western Blot: MDA5 Antibody (PSH03-56) [NBP3-32581] - Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (NBP3-32581) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Daudi cell lysate (20 ug/Lane) Lane 5: Raji cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: HeLa cell lysate (20 ug/Lane) Lane 8: SH-SY5Y cell lysate (20 ug/Lane) Lane 9: A549 cell lysate (20 ug/Lane) Lane 10: A431 cell lysate (20 ug/Lane) Lane 11: Mouse pancreas tissue lysate (40 ug/Lane) Lane 12: Rat kidney tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32581) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: MDA5 Antibody (1B6) [NBP1-97526] - Analysis of MDA5 monoclonal antibody (1B6) : Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.](http://images.novusbio.com/fullsize/MDA5-Antibody-1B6-Western-Blot-NBP1-97526-img0001.jpg "Western Blot: MDA5 Antibody (1B6) [NBP1-97526] - Analysis of MDA5 monoclonal antibody (1B6) : Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.")
![Western Blot: MDA5 Antibody (3D12) [NBP3-15166] - Positive WB detected in: THP-1 whole cell lysate, Mouse lung tissue. All lanes: MDA5 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 117, 26 KDa. Observed band size: 117 KDa.](http://images.novusbio.com/fullsize/MDA5-Antibody-3D12-Western-Blot-NBP3-15166-img0001.jpg "Western Blot: MDA5 Antibody (3D12) [NBP3-15166] - Positive WB detected in: THP-1 whole cell lysate, Mouse lung tissue. All lanes: MDA5 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 117, 26 KDa. Observed band size: 117 KDa.")
![Western Blot: MDA5 Antibody (8C10) [NBP2-46022] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDA5.](http://images.novusbio.com/fullsize/MDA5-Antibody-8C10-Western-Blot-NBP2-46022-img0001.jpg "Western Blot: MDA5 Antibody (8C10) [NBP2-46022] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDA5.")