![Western Blot: MBP Antibody [NB110-79873] - WB analysis of Myelin Basic Protein (MBP) in 10ug of normal brain lysate with antibody NB110-79873 at 1:500 dilution.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NB110-79873-img0001.jpg "Western Blot: MBP Antibody [NB110-79873] - WB analysis of Myelin Basic Protein (MBP) in 10ug of normal brain lysate with antibody NB110-79873 at 1:500 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ELISA, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Reacts with residues 49-62 of Isoforms 5 and 6 of the human protein. The immunogen corresponds to Isoform 1 between amino acids 182-195 of the 33 kDa isoform. The antibody also detects mouse and rat MBP protein. The antibody primarily detects a 22 kDa band. |
| Marker | Oligodendrocyte Marker, Myelin Marker |
| Specificity | Myelin Basic Protein, Reacts with residues 49-62 of the 33 kDa human, mouse and rat MBP protein. Antibody detects primarily the 22 kDa isoform. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MBP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is useful in ELISA, Immunocytochemistry, Immunohistochemistry 1:100-1:200 and Western Blot. In WB a band is seen at approx. 22 kDa representing MBP. Use in Immunohistochemistry free-floating reported in scientific literature (PMID 26616873). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MBP Antibody (NB110-79873)Find related products by research area.
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![Simple Western: MBP Antibody [NB110-79873] - Simple Western lane view shows a specific band for MBP in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image generated by internal validation.](http://images.novusbio.com/fullsize/antibody/nb110-79873_rabbit-polyclonal-mbp-antibody-simple-western-2382023114246..jpg "Simple Western: MBP Antibody [NB110-79873] - Simple Western lane view shows a specific band for MBP in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image generated by internal validation.")
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