Home » MAP3K9 » MAP3K9 Lysates » MAP3K9 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

MAP3K9 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: MAP3K9 Overexpression Lysate (Adult Normal) [NBP2-05914] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAP3K9.
Western Blot: MAP3K9 Overexpression Lysate (Adult Normal) [NBP2-05914] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAP3K9.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

MAP3K9 Overexpression Lysate Summary

Description

MAP3K9 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC215441

Accession#: NM_033141

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
MAP3K9

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
123.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for MAP3K9 Overexpression Lysate

  • EC 2.7.11
  • EC 2.7.11.25
  • MEKK9
  • mitogen-activated protein kinase kinase kinase 9
  • MLK1Mixed lineage kinase 1
  • PRKE1mixed lineage kinase 1 (tyr and ser/thr specificity)

Background

MLK1 is a MLK(MAP3K) type protein kinase. In vitro studies suggest that it is a member of the neuronal apoptotic JNK/c-Jun pathway acting between Rac1/Cdc42 and MKK4 and -7 in death signaling. MLK1 expression has been documented in human epithelial tumor cell lines of colonic, breast and esophageal origin. ESTs have been isolated from several tissue libraries, including normal pancreas and spleen and cancerous pancreas and bladder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MLK3 Antibody
AF4559
Western Blot MLK3 Antibody
Species: Hu
Applications: WB
MAP3K13 Antibody
NBP1-81272
Western Blot MAP3K13 Antibody
Immunohistochemistry-Paraffin MAP3K13 Antibody
Immunohistochemistry-Paraffin MAP3K13 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
MLK2 Antibody
AF5066
Western Blot MLK2 Antibody
(2 Publications)
Species: Hu
Applications: WB
JNK1 Antibody
(OTI4E3)
NBP1-48284
Western Blot JNK1 Antibody (OTI4E3)
Immunocytochemistry/ Immunofluorescence JNK1 Antibody (OTI4E3)
Immunohistochemistry-Paraffin JNK1 Antibody (OTI4E3)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MKK7/MEK7 Antibody
(4E5) - BSA Free
NBP2-37542
ELISA MKK7/MEK7 Antibody (4E5) - BSA Free
Western Blot MKK7/MEK7 Antibody (4E5) - BSA Free
Immunocytochemistry/ Immunofluorescence MKK7/MEK7 Antibody (4E5) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAP3K12 Antibody
NBP2-17218
Western Blot MAP3K12 Antibody
Western Blot MAP3K12 Antibody
Immunohistochemistry-Paraffin MAP3K12 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
p38 alpha Antibody
[Unconjugated]
AF8691
Simple Western p38 alpha Antibody [Unconjugated]
Western Blot p38 alpha Antibody [Unconjugated]
Immunohistochemistry p38 alpha Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
SGSM3 Antibody
NBP2-13304
Immunohistochemistry-Paraffin SGSM3 Antibody
Immunocytochemistry/ Immunofluorescence SGSM3 Antibody
Western Blot SGSM3 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MKK4/MEK4 Antibody
(OTI8A8) - Azide and ...
NBP2-72710
Western Blot MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free
Flow Cytometry MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free
Flow Cytometry MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free
Species: Ca, Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry GRAP2 Antibody (OTI1G2)
Flow Cytometry GRAP2 Antibody (OTI1G2)
Western Blot GRAP2 Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AIMP2 Antibody
NBP1-81575
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Crk Antibody
(3G11E8) - BSA Free
NBP2-37568
ELISA Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
POLDIP2 Antibody
NBP1-68874
Western Blot POLDIP2 Antibody
Western Blot POLDIP2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
AHSA1 Antibody
(1A2-A8)
H00010598-M01
Western Blot AHSA1 Antibody (1A2-A8)
Sandwich ELISA AHSA1 Antibody (1A2-A8)
(6 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
DAPK3/ZIPK
Antibody (4H4C8) - BS
NBP2-52452
ELISA DAPK3/ZIPK Antibody (4H4C8) - BSA Free
Western Blot DAPK3/ZIPK Antibody (4H4C8) - BSA Free
Western Blot DAPK3/ZIPK Antibody (4H4C8) - BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
Pref-1/DLK1/FA1
Antibody [Unconjugate
AF8277
Western Blot Pref-1/DLK1/FA1 Antibody [Unconjugated]
Immunohistochemistry Pref-1/DLK1/FA1 Antibody [Unconjugated]
Flow Cytometry Pref-1/DLK1/FA1 Antibody [Unconjugated]
(7 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(63 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
MAP3K9
Overexpression Lysate
NBP2-05914
MAP3K9 Overexpression Lysate
Species: Hu
Applications: WB

Publications for MAP3K9 Lysate (NBP2-05914) (0)

There are no publications for MAP3K9 Lysate (NBP2-05914).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MAP3K9 Lysate (NBP2-05914) (0)

There are no reviews for MAP3K9 Lysate (NBP2-05914). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MAP3K9 Lysate (NBP2-05914) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MAP3K9 Products

Research Areas for MAP3K9 Lysate (NBP2-05914)

Find related products by research area.

Blogs on MAP3K9

There are no specific blogs for MAP3K9, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
EphB2 Antibody [Unconjugated]
AF467
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
p38 alpha Antibody [Unconjugated]
AF8691

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MAP3K9 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol MAP3K9