Western Blot: MafG Antibody [NBP2-15019] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MafG Antibody [NBP2-15019] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAFG protein stained by MAFG antibody diluted at 1:500. Red: phalloidin, a ...read more
Western Blot: MafG Antibody [NBP2-15019] - A.50 ug rat muscle lysate/extract, 12% SDS-PAGE. MAFG antibody dilution: 1:1000.The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary ...read more
Western Blot: MafG Antibody [NBP2-15019] - A.50 ug mouse muscle lysate/extract, 12% SDS-PAGE, MAFG antibody dilution: 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary ...read more
Immunoprecipitation: MafG Antibody [NBP2-15019] - Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 ug of MAFG antibody. Western blot analysis was performed using MAFG antibody EasyBlot ...read more
18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-15019 in the following applications:
Use in Mouse reported in scientific literature (PMID:33754066). Zebrafish reactivity reported in scientific literature (PMID: 27737822).
Packaging, Storage & Formulations
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified
Alternate Names for MafG Antibody
basic leucine zipper transcription factor MafG
hMAF
MafG
MGC13090
MGC20149
transcription factor MafG
v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G
v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
Background
Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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