Western Blot: MAD4 Antibody (OTI7F7) [NBP2-46060] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed.Equivalent amounts of cell lysates (5 ug per ...read more
Immunocytochemistry/ Immunofluorescence: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human keratinocytes, PFA-fixed, permabilisation: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary ...read more
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer ...read more
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA ...read more
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 ...read more
23.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4 using NBP2-46060 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
MAD4 is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
PFA-fixed cells, permabilisation step is PBS-Triton 0.1% 20 minutes at room temperature, primary antibody used at 1/250e in PBS-BSA 2%, secondary antiboy conjugated to Alexa594
Product General Protocols
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