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MAD4 Antibody (OTI7F7) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI7F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72567AF350
Alexa Fluor 405
NBP2-72567AF405
Alexa Fluor 488
NBP2-72567AF488
Alexa Fluor 532
NBP2-72567AF532
Alexa Fluor 594
NBP2-72567AF594
Alexa Fluor 647
NBP2-72567AF647
Alexa Fluor 700
NBP2-72567AF700
Alexa Fluor 750
NBP2-72567AF750
Allophycocyanin
NBP2-72567APC
Biotin
NBP2-72567B
DyLight 350
NBP2-72567UV
DyLight 405
NBP2-72567V
DyLight 488
NBP2-72567G
DyLight 550
NBP2-72567R
DyLight 594
NBP2-72567DL594
DyLight 650
NBP2-72567C
DyLight 680
NBP2-72567FR
DyLight 755
NBP2-72567IR
FITC
NBP2-72567F
HRP
NBP2-72567H
Janelia Fluor 646
NBP2-72567JF646
PE
NBP2-72567PE
PerCP
NBP2-72567PCP
Unconjugated
NBP2-46060
mFluor Violet 450 SE
NBP2-72567MFV450
mFluor Violet 500 SE
NBP2-72567MFV500
mFluor Violet 610 SE
NBP2-72567MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46060
Azide and BSA Free
NBP2-72567
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MAD4 Antibody (OTI7F7) [Janelia Fluor® 549] Summary

Immunogen
Full length recombinant protein of human MAD4 (NP_006445) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MXD4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MAD4 Antibody (OTI7F7) [Janelia Fluor® 549]

  • BHLHC12
  • bHLHc12Max-interacting transcriptional repressor MAD4
  • Class C basic helix-loop-helix protein 12
  • Mad4 homolog
  • MAD4Max-associated protein 4
  • MAX dimerization protein 4
  • Max dimerizer 4
  • MST149
  • MSTP149
  • MXD4 MAX dimerization protein 4

Background

MAD4 is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MXD4