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Lysozyme Antibody

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Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin ...read more
Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody.Lane 1: Lysozyme (Hen Egg White).Load: 50 ng per lane.Primary antibody: Lysozyme antibody at 1:1,000 overnight at 4C.Secondary antibody: ...read more
ELISA: Lysozyme Antibody [NBP1-77790] - ELISA Results of Lysozyme antibody. Each well was coated in duplicate with 1.0 ug of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5ug/ml and the ...read more

Product Details

Summary
Reactivity ChSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price
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Lysozyme Antibody Summary

Description
This purified antibody is directed against lysozyme from hen egg white protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White)

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
This purified Lysozyme Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme. (Uniprot: Q6LEL2)
Marker
Histiocytic Marker
Specificity
This purified antibody is directed against lysozyme from hen egg white protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LYZ
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:25000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
Application Notes
This product has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.
Publications
Read Publication using NBP1-77790.

Reactivity Notes

A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Alternate Names for Lysozyme Antibody

  • EC 3.2.1.17
  • lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C
  • lysozyme C
  • Lysozyme
  • LZM
  • renal amyloidosis

Background

Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysozyme Antibody (NBP1-77790)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Lysozyme Antibody (NBP1-77790). (Showing 1 - 1 of 1 FAQ).

  1. What is the concentration of the antibody in the 0.1ml volume?
    • The concentration of NBP1-77790 is 1.0mg/ml.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Lysozyme Antibody (NBP1-77790)

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Blogs on Lysozyme.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

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Bioinformatics

Gene Symbol LYZ
Uniprot