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Lysozyme Antibody [Biotin]

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Western Blot: Lysozyme Antibody [Biotin] [NB100-2005] - Lane 1:Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Predicted/Observed size: 4.9kDa, 5 kDa for Lysozyme. Other band(s): none.

Product Details

Summary
Reactivity ChSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

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Lysozyme Antibody [Biotin] Summary

Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]
Immunogen
This purified Lysozyme Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme. (Uniprot: Q6LEL2)
Marker
Histiocytic Marker
Specificity
This purified antibody is directed against lysozyme from hen egg white protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LYZ
Purity
Multi-step
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Applications/Dilutions

Dilutions
  • ELISA 1:20000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:2000-1:10000
Application Notes
This product has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
Readout System

Reactivity Notes

A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Multi-step

Alternate Names for Lysozyme Antibody [Biotin]

  • EC 3.2.1.17
  • lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C
  • lysozyme C
  • Lysozyme
  • LZM
  • renal amyloidosis

Background

Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LYZ
Uniprot