![Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody (M01), clone 4F6.Lane 1: PTPN22 transfected lysate(85.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-4F6-Western-Blot-H00026191-M01-img0006.jpg "Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody (M01), clone 4F6.Lane 1: PTPN22 transfected lysate(85.1 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, Mu, PoSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 4F6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | PTPN22 (AAH17785, 1 a.a. ~ 179 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKEAEKRKSDYIIRTLKVKFNSVSVILAHQTSLQNLFSQITPAHF |
| Specificity | PTPN22 (4F6) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PTPN22 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validaton and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 over-expressed 293 cell line, cotransfected with PTPN22 Validated Chimera RNAi ( Cat # H00026191-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTPN22 monoclonal antibody (M01), clone 4F6 (Cat # H00026191-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-4F6-Western-Blot-H00026191-M01-img0004.jpg "Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 over-expressed 293 cell line, cotransfected with PTPN22 Validated Chimera RNAi ( Cat # H00026191-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTPN22 monoclonal antibody (M01), clone 4F6 (Cat # H00026191-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Detection limit for recombinant GST tagged PTPN22 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-4F6-ELISA-H00026191-M01-img0007.jpg "ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Detection limit for recombinant GST tagged PTPN22 is 1 ng/ml as a capture antibody.")
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