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Lunatic Fringe Antibody (JE66-62)

Images

 
Western Blot: Lunatic Fringe Antibody (JE66-62) [NBP3-32530] - Western blot analysis of Lunatic Fringe on different lysates with Rabbit anti-Lunatic Fringe antibody (NBP3-32530) at 1/1,000 dilution. Lane 1: Hela cell ...read more
Immunocytochemistry/ Immunofluorescence: Lunatic Fringe Antibody (JE66-62) [NBP3-32530] - Immunocytochemistry analysis of HUVEC cells labeling Lunatic Fringe with Rabbit anti-Lunatic Fringe antibody (NBP3-32530) at ...read more
Flow Cytometry: Lunatic Fringe Antibody (JE66-62) [NBP3-32530] - Flow cytometric analysis of K-562 cells labeling Lunatic Fringe. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32530, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
JE66-62
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Lunatic Fringe Antibody (JE66-62) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Synthetic peptide within Human Lunatic Fringe aa 330-379 / 379. (Uniprot: Q8NES3)
Localization
Golgi apparatus membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LFNG
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:1000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Lunatic Fringe Antibody (JE66-62)

  • beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
  • EC 2.4.1.222
  • LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
  • LFNG
  • lunatic fringe (Drosophila) homolog
  • lunatic fringe homolog (Drosophila)
  • Lunatic Fringe
  • O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
  • SCDO3
  • SCDO3EC 2.4.1.222

Background

Lunatic Fringe encodes a member of the glycosyltransferase superfamily. The encoded protein is a single-pass type II Golgi membrane protein that functions as a fucose-specific glycosyltransferase, adding an N-acetylglucosamine to the fucose residue of a group of signaling receptors involved in regulating cell fate decisions during development. Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Alternatively spliced transcript variants that encode different isoforms have been described, however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LFNG