Mouse myeloma cell line NS0-derived recombinant human LIMPII lumenal loop Arg27-Thr432 Accession # Q14108
Specificity
Detects human LIMPII in ELISAs and Western blots. In sandwich immunoassays, approximately 6% cross-reactivity with recombinant mouse LIMPII is observed and less than 0.3% cross-reactivity with recombinant human (rh) SR-B1 and rhCD36 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SCARB2
Purity Statement
Antigen Affinity-purified
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for LIMPII/SR-B2 Antibody [Unconjugated]
AMRF
CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
CD36 antigen
CD36L2
CD36L2LIMP II
HLGP85
LGP85
LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
LIMPII
LIMPIICD36 antigen-like 2
LPG85
lysosome membrane protein 2
Lysosome membrane protein II
SCARB2
Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
scavenger receptor class B, member 2
SRB2
SR-B2
SR-BII
Background
LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and as CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1 - 3). SR-B/CD36 family members are type III integral membrane proteins with an N- as well as a C-terminal cytoplasmic tail, and a large extracellular (or lumenal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is widely expressed on all tissues and cell types so far examined. It is also expressed on the surface of activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII-thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation (1). Overexpression of LIMPII causes an enlargement of early endosomes and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).
Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855.
Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
Eskelinen, E-L. et al. (2003) Trends in Cell Biology 13:137.
Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
Gamp, A-C. et al. (2003) Human Molecular Genetics 12:631.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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