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LHX2 Antibody (JE54-99)

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Western Blot: LHX2 Antibody (JE54-99) [NBP3-32533] - Western blot analysis of LHX2 on different lysates with Rabbit anti-LHX2 antibody (NBP3-32533) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: Neuro-2a cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 40-44 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32533) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Western Blot: LHX2 Antibody (JE54-99) [NBP3-32533] - Western blot analysis of LHX2 on different lysates with Rabbit anti-LHX2 antibody (NBP3-32533) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
JE54-99
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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LHX2 Antibody (JE54-99) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein within Human LHX2 aa 1-100 / 406. (Uniprot: P50458)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LHX2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for LHX2 Antibody (JE54-99)

  • hLhx2
  • Homeobox protein LH-2
  • LH-2
  • LH2MGC138390
  • LIM homeobox 2
  • LIM homeobox protein 2
  • LIM HOX gene 2
  • LIM/homeobox protein Lhx2

Background

LHX2 encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-32533
LHX2 Antibody (JE54-99)
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Bioinformatics

Gene Symbol LHX2