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LDB2 Antibody (PCRP-LDB2-1B10)

Images

 
Flow Cytometric Analysis of PFA-fixed HeLa cells. LDB2 antibody (PCRP-LDB2-1B10) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Formalin-fixed, paraffin-embedded human tonsil stained with LDB2 antibody (PCRP-LDB2-1B10). Inset: PBS instead of primary antibody; secondary only negative control.
Analysis of Protein Array containing more than 19,000 full-length human proteins using LDB2 antibody (PCRP-LDB2-1B10)
Flow Cytometric Analysis of PFA-fixed HeLa cells. LDB2 antibody (PCRP-LDB2-1B10) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Formalin-fixed, paraffin-embedded human papillary thyroid cancer stained with LDB2 antibody (PCRP-LDB2-1B10). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
PCRP-LDB2-1B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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LDB2 Antibody (PCRP-LDB2-1B10) Summary

Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Immunogen
Recombinant full-length human LDB2 protein
Localization
Nucleus.
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LDB2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for LDB2 Antibody (PCRP-LDB2-1B10)

  • Carboxyl-terminal LIM domain-binding protein 1
  • CLIM-1
  • CLIM1LDB1
  • LDB-2
  • LIM binding domain 2
  • LIM domain binding 2
  • LIM domain-binding factor CLIM1
  • LIM domain-binding factor-2
  • LIM domain-binding protein 2

Background

LDB2 is a transcriptional activator that associates with the LIM homeoproteins and coordinates transcription. LIM homeoproteins and LDBs are involved in a variety of developmental processes. LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors which might implicate them in human phenotypes characterized by multiple affected sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LDB2