I have been looking for anti PLOD2 antibody to perform IHC on human lung cancer tissue. I have tried some antibodies from different companies, but it did not work for us well. I found this antibody cat# H00005352-B01P at Novus Biologicals website. On the data sheet you reported it for ICC and IF. I have been using your different antibodies in the past and had some satisfying results. May I ask you, please, if you had tried IHC on human tissue and it did not work or you have not tried it at all?

Name: Lysine Hydroxylase 2/PLOD2 Antibody
Brand: Novus Biologicals
SKU: H00005352-B01P
Rating: 5 (1 reviews)

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H00005352-B01P is a product we distribute for a Taiwanese company called Abnova. We therefore do not do any in-house testing or development of their products. Abnova has validated this PLOD2 antibody for use in western via detection of the recombinant protein and overexpression transfected lysates. They have also validated this antibody in ICC with HeLa cells. They have not yet tested this antibody in tissue samples. See their datasheet here for all information we have regarding this product. Since this is not our product, we unfortunately cannot offer free samples. If you did want to try this product in an IHC experiment, we could offer you our Innovator's Reward Program. In exchange for a review of the product in an untested application or species, we would issue you a credit for the purchase price of the antibody for use in a future purchase. See details here Innovators Reward Program.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, ICC/IF
Clonality	Polyclonal
Host	Mouse
Conjugate	Unconjugated

Description	Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen	PLOD2 (NP_891988.1, 1 a.a. - 758 a.a.) full-length human protein. MGGCTVKPQLLLLALVLHPWNPCLGADSEKPSSIPTDKLLVITVATKESDGFHRFMQSAKYFNYTVKVLGQGEEWRGGDGINSIGGGQKVRLMKEVMEHYADQDDLVVMFTECFDVIFAGGPEEVLKKFQKANHKVVFAADGILWPDKRLADKYPVVHIGKRYLNSGGFIGYAPYVNRIVQQWNLQDNDDDQLFYTKVYIDPLKREAINITLDHKCKIFQTLNGAVDEVVLKFENGKARAKNTFYETLPVAINGNGPTKILLNYFGNYVPNSWTQDNGCTLCEFDTVDLSAVDVHPNVSIGVFIEQPTPFLPRFLDILLTLDYPKEALKLFIHNKEVYHEKDIKVFFDKAKHEIKTIKIVGPEENLSQAEARNMGMDFCRQDEKCDYYFSVDADVVLTNPRTLKILIEQNRKIIAPLVTRHGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQPCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHMKQVDLENVWLHFIREFIAPVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTFTINIALNNVGEDFQGGGCKFLRYNCSIESPRKGWSFMHPGRLTHLHEGLPVKNGTRYIAVSFIDP
Specificity	PLOD2 - procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2,
Isotype	IgG
Clonality	Polyclonal
Host	Mouse
Gene	PLOD2
Purity	IgG purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7.4)
Preservative	No Preservative
Purity	IgG purified

Publications using H00005352-B01P	Applications	Species
Labrecque MP, Takhar MK, Nason R et al. The retinoblastoma protein regulates hypoxia-inducible genetic programs, tumor cell invasiveness and neuroendocrine differentiation in prostate cancer cells. Oncotarget 2016-04-26 [PMID: 27015368] (WB)	WB
Labrecque Mark P, Takhar Mandeep K, Jagdeo Julienne M et al. A TRIP230-retinoblastoma protein complex regulates hypoxia-inducible factor-1alpha-mediated transcription and cancer cell invasion. PLoS One. 2014-06-11 [PMID: 24919196] (Human)		Human
Gilkes DM, Bajpai S, Wong CC et al. Procollagen lysyl hydroxylase 2 is essential for hypoxia-induced breast cancer metastasis. Mol Cancer Res 2013-05-01 [PMID: 23378577]

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Gene Symbol	PLOD2
Entrez	5352(Human)
Uniprot	NP_891988.1(Human)

	Verified Customer 01/22/2010
Application:	WB
Species:	Human