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Lck Antibody [Unconjugated]

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Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human Lck Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3704) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Lck at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human Lck Antigen ...read more
Lck was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human Lck Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3704) at 3 µg/mL for 1 hour at room ...read more
TIM-3 Association with Intracellular Kinases in CD8+/MART-1+ T cells.TIM-3 co-immunoprecipitation analysis of unactivated and 15 min. stimulation with anti-CD3/CD28 beads (activated). Equivalent amounts of protein ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Lck at approximately 57 kDa (as indicated) using 5 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3704-SP
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Lck Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Lck
Met1-Val66
Accession # P06239
Specificity
Detects human Lck in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LCK
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3704 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lck Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.2
  • Hck-3
  • Lck
  • Lcktkr
  • Leukocyte C-terminal Src kinase
  • LSK
  • LSTRA
  • Lymphocyte cell-specific protein-tyrosine kinase
  • lymphocyte-specific protein tyrosine kinase
  • p56(LSTRA) protein-tyrosine kinase
  • p56lck
  • p56-LCK
  • pp58lck
  • Protein YT16
  • Proto-oncogene Lck
  • proto-oncogene tyrosine-protein kinase LCK
  • T cell-specific protein-tyrosine kinase
  • T-lymphocyte specific protein tyrosine kinase p56lck
  • tyrosine-protein kinase Lck
  • YT16

Background

Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). Within aa 2-66, human Lck shares 89% aa sequence identity with mouse and rat Lck. A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lck Antibody (AF3704)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, Simple Western.


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(1)
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(1)
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(2)
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Bioinformatics

Gene Symbol LCK
Uniprot