![Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-OTI3G8-Western-Blot-NBP2-02340-img0010.jpg "Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3G8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human IFIT1 (NP_000899) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IFIT1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | For IHC-P, we recommend using Tris/EDTA pH 8.0 heat induced epitope retrieval. |
|
| Theoretical MW | 55.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-02340 | Applications | Species |
|---|---|---|
| Johnson MB, Furr KH, Suptela SR et al. Induction of protective interferon-b responses in murine osteoblasts following Staphylococcus aureus infection Frontiers in microbiology 2022-12-02 [PMID: 36532419] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for IFIT1 Antibody (NBP2-02340)Find related products by research area.
|
|
STING in Innate Immunity and Cancer: What’s the Buzz About? STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02340-img0008.jpg "Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.")
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human liver tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0007.jpg "Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human liver tissue using anti-IFIT1 mouse monoclonal antibody.")
![Flow Cytometry: IFIT1 Antibody (OTI3G8) [NBP2-02340] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFIT1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Flow-Cytometry-NBP2-02340-img0001.jpg "Flow Cytometry: IFIT1 Antibody (OTI3G8) [NBP2-02340] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFIT1 antibody, and then analyzed by flow cytometry.")
![Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT1.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Western-Blot-NBP2-02340-img0002.jpg "Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT1.")
![Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Western-Blot-NBP2-02340-img0009.jpg "Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Immunofluorescent staining of HT29 cells using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02340-img0003.jpg "Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Immunofluorescent staining of HT29 cells using anti-IFIT1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0004.jpg "Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IFIT1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0005.jpg "Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IFIT1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human Kidney tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0006.jpg "Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human Kidney tissue using anti-IFIT1 mouse monoclonal antibody.")
![Immunocytochemistry Lck [p Tyr394] Antibody (755103)](https://images.novusbio.com/images2/Lck_MAB7500_Immunocytochemistry_12319.jpg)
![Western Blot Lck [p Tyr394] Antibody (755103)](https://images.novusbio.com/images2/Lck_MAB7500_Western_Blot_12455.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![ISG15/UCRP [7-Amino-4-Methyl Coumarin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "ISG15/UCRP [7-Amino-4-Methyl Coumarin]")
![Western Blot Lyn Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyn_AF3206_Western_Blot_5256.jpg)
![Simple Western Lyn Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyn_AF3206_Simple_Western_21252.jpg)
![Immunocytochemistry Lyn Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyn_AF3206_Immunocytochemistry_9784.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_723.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_724.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/CXCL10_DIP100_ELISA_121.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")