LAMP-1/CD107a Antibody (5E7) - BSA Free

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Zebrafish.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Showing Publications 1 - 7 of 7.

Publications using NBP2-52721	Applications	Species
Chen Q, Cao Y, Li H et al. Sodium nitroprusside alleviates nanoplastics-induced developmental toxicity by suppressing apoptosis, ferroptosis and inflammation Journal of environmental management 2023-08-01 [PMID: 37536135] (WB, Zebrafish)	WB	Zebrafish
Alban TJ, Grabowski MM, Otvos B et al. The MIF promoter SNP rs755622 is associated with immune activation in glioblastoma JCI insight 2023-07-10 [PMID: 37252795] (IHC-P, Human) Details: 1:50 IHC-P dilution	IHC-P	Human
Larionova I, Kiselev A, Kazakova E et al. Tumor-associated macrophages respond to chemotherapy by detrimental transcriptional reprogramming and suppressing stabilin-1 mediated clearance of EGF Frontiers in immunology 2023-03-07 [PMID: 36960065] (ICC/IF, Human) Details: Dilution used in IHC-P 1:100	ICC/IF	Human
Zhang G, Cong Y, Liu FL et al. A nanomaterial targeting the spike protein captures SARS-CoV-2 variants and promotes viral elimination Nature nanotechnology 2022-08-22 [PMID: 35995853] (ICC/IF, Human)	ICC/IF	Human
Yoon Y, Go G, Yoon S Et Al. Melatonin Treatment Improves Renal Fibrosis via miR-4516/SIAH3/PINK1 Axis Cells 2021-07-03 [PMID: 34359852]
Boukhedouni N, Martins C, Darrigade AS et al. Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo JCI Insight 2020-06-04 [PMID: 32369451] (IHC-P, Human)	IHC-P	Human
Koupenova M, Corkrey HA, Vitseva O et al. The role of platelets in mediating a response to human influenza infection. Nat Commun. 2018-09-01 [PMID: 30992428] (ICC/IF, Human) Details: Citation using the Azide Free form of this antibody.	ICC/IF	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	ICC	Human	10/27/2018	View Summary Application Immunocytochemistry Sample Tested MDA MB 231 cells Species Human Lot A-1
Enlarge	5 reviewed by: Verified Customer	ICC	Human	06/28/2018	View Summary Application Immunocytochemistry Sample Tested MCF-7 human breast cancer cell line Species Human Lot a1 Comments Comments Secondary Antibody Dy680

Array NBP2-52721

• LAMP-1/CD107a Antibodies
• LAMP-1/CD107a Antibody Pair
• LAMP-1/CD107a Lysate
• LAMP-1/CD107a Proteins
• LAMP-1/CD107a Proteins and Enzymes