![Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Western-Blot-NBP1-97687-img0003.jpg "Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Ha, ShSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 131C3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Immunogen | Derived by fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with purified Rat liver lamins. |
| Marker | Nuclear Envelope Marker |
| Specificity | This antibody reacts with an epitope located between residues 319-566 in lamin A and C. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LMNA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications. This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.2 |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lamin A + C Antibody (NBP1-97687)Find related products by research area.
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| Gene Symbol | LMNA |
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry on frozen tissue section of human skin showing nuclear lamina staining in epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0004.jpg "Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry on frozen tissue section of human skin showing nuclear lamina staining in epithelial and connective tissue cells.")
![Immunohistochemistry: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of on frozen sections of swine skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-NBP1-97687-img0001.jpg "Immunohistochemistry: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of on frozen sections of swine skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.")
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of NBP1-97687(131C3) on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0002.jpg "Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of NBP1-97687(131C3) on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")