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L2HGDH Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: L2HGDH Antibody [NBP2-49445] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
Immunohistochemistry-Paraffin: L2HGDH Antibody [NBP2-49445] - Staining of human duodenum shows strong positivity with a granular a pattern in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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L2HGDH Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KKEPYCRGLMAIDCPHTGIVDYRQVALSFAQDFQEAGGSVLTNFEVKGIEMAKESPSRSIDGMQYPIVIKNTKGEEI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
L2HGDH
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
L2HGDH Recombinant Protein Antigen (NBP2-49445PEP)

Reactivity Notes

Mouse (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for L2HGDH Antibody

  • 2-hydroxyglutarate dehydrogenase
  • alpha-ketoglutarate reductase
  • C14orf160alpha-hydroxyglutarate oxidoreductase
  • chromosome 14 open reading frame 160
  • DURANIN
  • EC 1.1.99.2
  • FLJ12618
  • L-2-hydroxyglutarate dehydrogenase
  • L-2-hydroxyglutarate dehydrogenase, mitochondrial
  • L-alpha-hydroxyglutarate dehydrogenase

Background

The L2HGDH gene encodes L-2-hydroxyglutarate dehydrogenase, a FAD-dependent enzyme that oxidizes L-2-hydroxyglutarate to alpha-ketoglutarate in a variety of mammalian tissues. Mutations in this gene cause L-2-hydroxyglutaric aciduria, a rare autosomal recessive

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol L2HGDH